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Polycystin 2 antibody

Orthogonal
Anti-Polycystin 2 antibody used in Western Blot (WB). GTX113802
Anti-Polycystin 2 antibody used in Western Blot (WB). GTX113802
Anti-Polycystin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113802
Anti-Polycystin 2 antibody used in IHC (Whole mount) (IHC-Wm). GTX113802

Cat. No. GTX113802

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Polycystin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human Polycystin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165095

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polycystin 2, transient receptor potential cation channel , APKD2 , PC2 , PKD4 , Pc-2 , TRPP2

Cellular Localization

Membrane; Multi-pass membrane protein

Background

This gene encodes a member of the polycystin protein family. The encoded protein contains multiple transmembrane domains, and cytoplasmic N- and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. The encoded protein may function in renal tubular development, morphology, and function, and may modulate intracellular calcium homoeostasis and other signal transduction pathways. This protein interacts with polycystin 1 to produce cation-permeable currents. Mutations in this gene have been associated with autosomal dominant polycystic kidney disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Polycystin 2 antibody used in Western Blot (WB). GTX113802

GTX113802 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Polycystin 2 antibody (GTX113802) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Polycystin 2 antibody used in Western Blot (WB). GTX113802

GTX113802 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX113802 diluted at 1:500.

Anti-Polycystin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113802

GTX113802 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using Polycystin 2(GTX113802) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Polycystin 2 antibody used in IHC (Whole mount) (IHC-Wm). GTX113802

GTX113802 IHC-Wm Image

The data was published in the journal Biol Open in 2015. PMID: 26432887

REFERENCE

Application Reference

In?s Oliveira et al. Int J Mol Sci 2021; 22 (16) Zebrafish Model as a Screen to Prevent Cyst Inflation in Autosomal Dominant Polycystic Kidney Disease.
Application : IHC-Wm
Reactivity : Zebrafish
Roxo-Rosa M et al. Biol Open 2015; 4 (11) : 1356-66 The zebrafish Kupffer's vesicle as a model system for the molecular mechanisms by which the lack of Polycystin-2 leads to stimulation of CFTR.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169