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Polycystin 2 antibody

Anti-Polycystin 2 antibody used in Western Blot (WB). GTX54867
Anti-Polycystin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54867
Anti-Polycystin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54867

Cat. No. GTX54867

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all Polycystin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)ERWESDDAASQISH, corresponding to amino acid residues 914-927 (Intracellular, C-terminus) of human TRPP1 (Accession  : Q13563).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polycystin 2, transient receptor potential cation channel , APKD2 , PC2 , PKD4 , Pc-2 , TRPP2

Cellular Localization

Endoplasmic reticulum membrane,Cell membrane,Golgi apparatus

Background

This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-Polycystin 2 antibody used in Western Blot (WB). GTX54867

GTX54867 WB Image

WB analysis of rat kidney membrane (lanes 1 and 3) and mouse kidney (lanes 2 and 4) lysates using GTX54867 Polycystin 2 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-Polycystin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54867

GTX54867 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX54867 Polycystin 2 antibody. (PT = proximal tubule, DT = distal tubule)
Panel A : TRPP1 (red) appears in the renal cortex, mainly in the distal tubules
Panel B : DAB staining of TRPP1 (brown) also shows that the renal corpuscle and the blood vessels are not stained at all.
Dilution : 1:100

Anti-Polycystin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54867

GTX54867 ICC/IF Image

ICC/IF analysis of PFA-fixed C2C12 cells using GTX54867 Polycystin 2 antibody.
Panel A : Primary antibody
Panel B : DNA dye Hoechst 33342
Panel C : Merged images of panels A and B
Dilution : 1:50-1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639