Mouse plasma (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Prealbumin antibody [N1C3] (GTX100577) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat plasma (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Prealbumin antibody [N1C3] (GTX100577) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human plasma (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Prealbumin antibody [N1C3] (GTX100577) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.