Wild-type (WT) and Presenilin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Presenilin 1 antibody [C1C2], Internal (GTX116016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Presenilin 1 antibody [C1C2], Internal detects Presenilin 1 protein at Golgi apparatus and endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Presenilin 1 stained by Presenilin 1 antibody [C1C2], Internal (GTX116016) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Presenilin 1 antibody [C1C2], Internal (GTX116016) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Presenilin 1(GTX116016) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Presenilin 1 antibody [C1C2], Internal (GTX116016) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min