Various tissue extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PDYN antibody [HL2573] (GTX638951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ProDynorphin antibody [HL2573] (GTX638951) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.