Various tissue extracts (20 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ProDynorphin antibody [HL2574] (GTX638952) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ProDynorphin antibody [HL2574] (GTX638952) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PDYN antibody [HL2574] detects secreted PDYN protein by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
PDYN stained by PDYN antibody [HL2574] (GTX638952) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min