Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX108589 diluted at 1:1000

Sample(30 ug whole cell lysate)
A: A431(GTX27909)
B: H1299
12% SDS PAGE
GTX108589 diluted at 1:500

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 2 antibody (GTX108589) diluted at 1:1000.

Profilin 2 antibody detects PFN2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
Profilin 2 antibody (GTX108589) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min