WB analysis of 20 ug of nuclear (N) or cytoplasmic (C) of T47D cell lysate untreated (-) or stimulated (+) with 100 nM promegestone (R5020) for 1 hour using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154] at 4 μg/ml (upper panel) or a Progesterone Receptor mouse monoclonal antibody (lower pane) . This antibody detects both phosphorylated isoform A and isoform B.

ICC/IF analysis of T47D cells using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

IHC-P analysis of human uterus tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

IHC-P analysis of human tonsil tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

IHC-P analysis of human breast carcinoma tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

ICC/IF analysis of untreated T47D cells (left panel) or stimulated cells with 100 nM of Progesterone (right panel) using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute