Sample (30 ug of whole cell lysate)
A: IMR32
B: U87-MG
12% SDS PAGE
GTX113243 diluted at 1:1000

Prohibitin antibody detects Prohibitin protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Prohibitin antibody (GTX113243) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: Prohibitin protein stained by Prohibitin antibody (GTX113243) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Prohibitin antibody detects Prohibitin protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Prohibitin antibody (GTX113243) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Prohibitin antibody detects PHB protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
Prohibitin antibody (GTX113243) dilution: 1:1000

Prohibitin antibody detects PHB protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
12 % SDS-PAGE
Prohibitin antibody (GTX113243) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Prohibitin(GTX113243) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min