Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody (GTX101199) diluted at 1:500.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody (GTX101199) diluted at 1:500.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
7.5% SDS PAGE
GTX101199 diluted at 1:1000

Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Prothrombin antibody (GTX101199) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.