GTX119726 IP Image
pygopus 2 was immunoprecipitated from 5*107 cells of SW480 whole cell lysate using 1 ug of anti-pygopus 2 antibody (GTX119726).
The precipitated pygopus 2 was detected by GTX119726 diluted at 1:15000. A: IP-IgG

B: IP-pygopus 2 antibody

Pygopus 2 antibody detects Pygopus 2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pygopus 2 protein stained by Pygopus 2 antibody (GTX119726) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Pygopus 2 antibody detects Pygopus 2 protein at nucleus in mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Pygopus 2 antibody (GTX119726) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pygopus 2 antibody (GTX119726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pygopus 2 antibody (GTX119726) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Pygopus 2 antibody detects Pygopus 2 protein at nucleus in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Pygopus 2 antibody (GTX119726) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min