Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

RAB35 antibody [N1C3] detects RAB35 protein at Golgi apparatus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB35 stained by RAB35 antibody [N1C3] (GTX120294) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using RAB35(GTX120294) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RAB35 stained by RAB35 antibody [N1C3] (GTX120294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
RAB35 stained by RAB35 antibody [N1C3] (GTX120294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min