Wild-type (WT) and RAB6A knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody (GTX110646) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using RAB6A(GTX110646) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

RAB6A antibody detects RAB6A protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
RAB6A antibody (GTX110646) dilution: 1:1000

RAB6A antibody detects RAB6A protein at Golgi apparatus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB6A protein stained by RAB6A antibody (GTX110646) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

RAB6A antibody detects RAB6A protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
12 % SDS-PAGE
RAB6A antibody (GTX110646) dilution: 1:1000

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RAB6A(GTX110646) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody (GTX110646) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Sci Rep in 2017.PMID: 29127306

The data was published in the journal Elife in 2015. PMID: 25821985