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RAB6A antibody

KO/KD Orthogonal
Anti-RAB6A antibody used in Western Blot (WB). GTX110646
Anti-RAB6A antibody used in Western Blot (WB). GTX110646
Anti-RAB6A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110646
Anti-RAB6A antibody used in Western Blot (WB). GTX110646
Anti-RAB6A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110646
Anti-RAB6A antibody used in Western Blot (WB). GTX110646
Anti-RAB6A antibody used in IHC (Paraffin sections) (IHC-P). GTX110646
Anti-RAB6A antibody used in Western Blot (WB). GTX110646
Anti-RAB6A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110646

Cat. No. GTX110646

Reference 5
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RAB6A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

mouse brain , rat brain , 293T

Predict Reactivity

Zebrafish, Drosophila, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human RAB6A Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951582

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB6A, member RAS oncogene family , RAB6

Cellular Localization

Golgi apparatus membrane; Lipid-anchor

Background

Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity.

Database

Research Area

DATA IMAGES

Anti-RAB6A antibody used in Western Blot (WB). GTX110646

GTX110646 WB Image

KO/KD

Wild-type (WT) and RAB6A knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody (GTX110646) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB6A antibody used in Western Blot (WB). GTX110646

GTX110646 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody (GTX110646) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB6A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110646

GTX110646 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RAB6A(GTX110646) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-RAB6A antibody used in Western Blot (WB). GTX110646

GTX110646 WB Image

RAB6A antibody detects RAB6A protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
12 % SDS-PAGE
RAB6A antibody (GTX110646) dilution: 1:1000

Anti-RAB6A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110646

GTX110646 ICC/IF Image

RAB6A antibody detects RAB6A protein at Golgi apparatus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB6A protein stained by RAB6A antibody (GTX110646) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RAB6A antibody used in Western Blot (WB). GTX110646

GTX110646 WB Image

RAB6A antibody detects RAB6A protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
RAB6A antibody (GTX110646) dilution: 1:1000

Anti-RAB6A antibody used in IHC (Paraffin sections) (IHC-P). GTX110646

GTX110646 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using RAB6A(GTX110646) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAB6A antibody used in Western Blot (WB). GTX110646

GTX110646 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017.PMID: 29127306

Anti-RAB6A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110646

GTX110646 ICC/IF Image

The data was published in the journal Elife in 2015. PMID: 25821985

REFERENCE

Application Reference

Kawabata M et al. PLoS Pathog 2021; 17 (3) : e1009437 Legionella hijacks the host Golgi-to-ER retrograde pathway for the association of Legionella-containing vacuole with the ER.
Application : ICC/IF
Reactivity : Human
Da Costa R et al. Cell Mol Life Sci 2020; 77 (3) : 511-529 Vps13b is required for acrosome biogenesis through functions in Golgi dynamic and membrane trafficking.
Ishida M et al. J Cell Biol 2019; ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network.
Application : WB
Reactivity : Human
Wu CH et al. Sci Rep 2017; 7 (1) : 15320 Upregulation of microRNA-4417 and Its Target Genes Contribute to Nickel Chloride-promoted Lung Epithelial Cell Fibrogenesis and Tumorigenesis.
Lee PL et al. Elife 2015; 4 Rab6 regulation of the kinesin family KIF1C motor domain contributes to Golgi tethering.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

RAB6A antibody Cat. No. GTX110646

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-RAB6A antibody used in Immunoprecipitation (IP). GTX110646
Date : Anonymous submitted on 08-Jul-2024
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description :
SM=4% starting material
UB=4% unbound fraction
Figure Note :
Antibody-beads conjugates were prepared by adding 2 μg to 500 μl of IP Lysis Buffer in a microcentrifuge tube, together with with 30 μl of Dynabeads protein A- (for rabbit antibodies) or protein G- (for mouse and rat antibodies) beads.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-RAB6A antibody used in Western Blot (WB). GTX110646
Date : Anonymous submitted on 08-Jul-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and RAB6A KO cells
Amount used : 40μg
Blocking : 5% milk, 1Hr
Primary Antibody : 1:1000, 4°C, >12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169