Wild-type (WT) and hHR23b knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hHR23b antibody (GTX113820) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using RAD23B(GTX113820) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAD23B(GTX113820) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: A431
B: Jurkat
C: Raji
10% SDS PAGE
GTX113820 diluted at 1:1000