Sample (30 ug of whole cell lysate)
A:  Non-transfected 293T lysates 
B: C9orf102 transfected 293T lysates
10% SDS PAGE
GTX125944 diluted at 1:5000

C9orf102 antibody detects C9orf102 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
C9orf102 antibody (GTX125944) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX125944 diluted at 1:1000