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RAI3 antibody [HL1864]

Recombinant antibody KO/KD Comparison Orthogonal Overexpression
Anti-RAI3 antibody [HL1864] used in Western Blot (WB). GTX637589
Anti-RAI3 antibody [HL1864] used in Western Blot (WB). GTX637589
Anti-RAI3 antibody [HL1864] used in Western Blot (WB). GTX637589
Anti-RAI3 antibody [HL1864] used in IHC (Paraffin sections) (IHC-P). GTX637589
Anti-RAI3 antibody [HL1864] used in IHC (Paraffin sections) (IHC-P). GTX637589
Anti-RAI3 antibody [HL1864] used in IHC (Paraffin sections) (IHC-P). GTX637589
Anti-RAI3 antibody [HL1864] used in Western Blot (WB). GTX637589
Anti-RAI3 antibody [HL1864] used in Western Blot (WB). GTX637589
Anti-RAI3 antibody [HL1864] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637589

Cat. No. GTX637589

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1864

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RAI3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Product Note

This antibody was raised against human RAI3 Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human RAI3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor class C group 5 member A , GPCR5A , PEIG-1 , RAI3 , RAIG1 , TIG1

Cellular Localization

Cell membrane

Background

This gene encodes a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAI3 antibody [HL1864] used in Western Blot (WB). GTX637589

GTX637589 WB Image

KO/KD

Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAI3 antibody [HL1864] used in Western Blot (WB). GTX637589

GTX637589 WB Image

KO/KD Comparison

Non-transfected (–) and transfected (+) unbiled MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with RAI3 antibody [HL1864] (GTX637589) diluted at 1:5000 and the highly cited competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-RAI3 antibody [HL1864] used in Western Blot (WB). GTX637589

GTX637589 WB Image

KO/KD Comparison Orthogonal

Wild-type (WT) and RAI3 knockout (KO) MCF-7 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with RAI3 antibody [HL1864] (GTX637589) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-RAI3 antibody [HL1864] used in IHC (Paraffin sections) (IHC-P). GTX637589

GTX637589 IHC-P Image

Comparison

RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RAI3 stained by RAI3 antibody [HL1864] (GTX637589) diluted at 1:100 and competitor's antibody (10309-1-AP) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-RAI3 antibody [HL1864] used in IHC (Paraffin sections) (IHC-P). GTX637589

GTX637589 IHC-P Image

RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
RAI3 stained by RAI3 antibody [HL1864] (GTX637589) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAI3 antibody [HL1864] used in IHC (Paraffin sections) (IHC-P). GTX637589

GTX637589 IHC-P Image

RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
RAI3 stained by RAI3 antibody [HL1864] (GTX637589) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAI3 antibody [HL1864] used in Western Blot (WB). GTX637589

GTX637589 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RAI3 antibody [HL1864] used in Western Blot (WB). GTX637589

GTX637589 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAI3 antibody [HL1864] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637589

GTX637589 ICC/IF Image

RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: RAI3 stained by RAI3 antibody [HL1864] (GTX637589) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations