Boiled and unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL3393] (GTX641213) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL3393] (GTX641213) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL3393] (GTX641213) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GPRC5A antibody [HL3393] detects GPRC5A protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: GPRC5A stained by GPRC5A antibody [HL3393] (GTX641213) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).