Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAP1B antibody (GTX101950) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX101950 diluted at 1:500

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using RAP1B(GTX101950) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min