Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBP1 antibody (GTX108016) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

RBP1 antibody detects RBP1 protein at cytoplasm by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBP1 stained by RBP1 antibody (GTX108016) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Immunohistochemical analysis of paraffin-embedded human colon, using CRBP I(GTX108016) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RBP1 antibody (GTX108016) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.