Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RBP-Jkappa(GTX112358) antibody at 1:100 dilution.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RBPJK antibody [C1C3] (GTX112358) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody,