Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP antibody [GT1168] (GTX09128) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IP analysis of HeLa cell lysate using GTX09128 RIP antibody [GT1168].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:500

WB analysis of Raji and HeLa whole cell lysates using GTX09128 RIP antibody [GT1168].
Dilution : 1:1000
Loading : 25μg