HeLa whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody (GTX114103) diluted at 1:1000.

Sample (15 μg of whole cell lysate)
A: MDA-Mb-361
B: HCC1937 Lx ( Lentivirus infection, carry contol vector)
C: HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1)
GTX114103 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ROBO1 antibody detects ROBO1 protein at cytosol on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ROBO1 antibody (GTX114103) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ROBO1 antibody detects ROBO1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROBO1 stained by ROBO1 antibody (GTX114103) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody (GTX114103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.