ROCK1 antibody [N1N2], N-term

Cat. No. GTX113266
Cat. No.  GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
GTX113266 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
GTX113266 WB Image

Wild-type (WT) and ROCK1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
GTX113266 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

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Anti-ROCK1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX113266
GTX113266 IP Image

ROCK1 antibody immunoprecipitates ROCK1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of ROCK1 by 3 μg of ROCK1 antibody (GTX113266) 5% SDS-PAGE The immunoprecipitated ROCK1 protein was detected by ROCK1 antibody (GTX113266) diluted at 1 : 500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

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Anti-ROCK1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113266
GTX113266 ICC/IF Image

ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROCK1 protein stained by ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

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Anti-ROCK1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113266
GTX113266 ICC/IF Image

ROCK1 antibody [N1N2], N-term detects ROCK1 protein at nucleus and Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROCK1 stained by ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-ROCK1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113266
GTX113266 IHC-P Image

ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
GTX113266 WB Image

ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
5% SDS-PAGE
ROCK1 antibody [N1N2], N-term (GTX113266) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
GTX113266 WB Image

ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell extract
B. 30 μg C2Cl2 whole cell extract
5% SDS-PAGE
ROCK1 antibody [N1N2], N-term (GTX113266) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

9 / 10
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
GTX113266 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

10 / 10
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P IP
  • Reactivity
    Human, Mouse, Rat
See all ROCK1 products