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ROCK2 (phospho Ser1366) antibody

KO/KD Orthogonal Overexpression
Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122651

Cat. No. GTX122651

Reference 12

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, Immunoassay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ROCK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Immunoassay Assay dependent
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Positive Control

HeLa , 293T

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1366 of human ROCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2560946

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 2 , ROCK-II

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651

GTX122651 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651

GTX122651 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651

GTX122651 IHC-P Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 protein at nucleus and cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
ROCK2 (phospho Ser1366) antibody (GTX122651) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651

GTX122651 IHC-P Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 protein at weak nucleus and cytosol on human breast tissue by immunohistochemical analysis.
Sample: Paraffin-embedded human breast tissue.
ROCK2 (phospho Ser1366) antibody (GTX122651) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651

GTX122651 IHC-P Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651

GTX122651 IHC-P Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651

GTX122651 WB Image

Overexpression

ROCKII (phospho Ser1366) antibody  detects the phosphorylation of ROCKII at Ser 1366 residue in IP experiments.
HEK293T cells were transfected with wild type (WT) or phospho-mutant (S1366A) ROCKII expression constructs.
Overexpressed Myc-ROCKII proteins were immunoprecipitated with anti-myc antibody and probed with anti- ROCKII (phospho Ser1366) antibody (GTX122651, 1:1000 dilution) with or without peptide (0.2 mg/ml) competition as indicated.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651

GTX122651 IHC-P Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
ROCK2 (phospho Ser1366) stained by ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651

GTX122651 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ROCK2 (phospho Ser1366) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122651

GTX122651 ICC/IF Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ROCK2 (phospho Ser1366) protein stained by ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Sequeira MK et al. iScience 2023; 26 (4) : 106240 Cocaine and habit training cause dendritic spine rearrangement in the prelimbic cortex.
S?nchez-de la Torre A et al. Cell Death Dis 2022; 13 (7) : 585 Cannabinoid CB1 receptor gene inactivation in oligodendrocyte precursors disrupts oligodendrogenesis and myelination in mice.
Application : WB
Reactivity : Mouse
Huaqing Zheng et al. Acta Pharm Sin B 2021; 11 (12) : 3994-4007 TMEM16A inhibits angiotensin II-induced basilar artery smooth muscle cell migration in a WNK1-dependent manner.
Application : WB
Reactivity : Mouse
Roberta Romano et al. Cancers (Basel) 2021; 13 (9) RAB7A Regulates Vimentin Phosphorylation through AKT and PAK.
Application : WB
Reactivity : Human
Wei L et al. FASEB J 2020; 34 (1) : 474-493 ROCK2 inhibition enhances the thermogenic program in white and brown fat tissue in mice.
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REVIEW

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Application Reference
Sequeira MK et al. iScience 2023; 26 (4):106240 Cocaine and habit training cause dendritic spine rearrangement in the prelimbic cortex.
S?nchez-de la Torre A et al. Cell Death Dis 2022; 13 (7):585 Cannabinoid CB1 receptor gene inactivation in oligodendrocyte precursors disrupts oligodendrogenesis and myelination in mice.
Application : WB
Reactivity : Mouse
Huaqing Zheng et al. Acta Pharm Sin B 2021; 11 (12):3994-4007 TMEM16A inhibits angiotensin II-induced basilar artery smooth muscle cell migration in a WNK1-dependent manner.
Application : WB
Reactivity : Mouse
Roberta Romano et al. Cancers (Basel) 2021; 13 (9) RAB7A Regulates Vimentin Phosphorylation through AKT and PAK.
Application : WB
Reactivity : Human
Wei L et al. FASEB J 2020; 34 (1):474-493 ROCK2 inhibition enhances the thermogenic program in white and brown fat tissue in mice.
Marceaux C et al. J Cell Sci 2018; (Epub) Phosphorylation of ARHGAP19 by CDK1 and ROCK regulates its subcellular localization and function during mitosis.
Application : WB
Reactivity : Human
Lu DH et al. J Thromb Haemost 2017; (Epub) ARHGEF10 knockout inhibits platelet aggregation and protects mice from thrombus formation.
Application : WB
Reactivity : Mouse
Wang W et al. Cereb Cortex 2017;:43114 Atypical Endocannabinoid Signaling Initiates a New Form of Memory-Related Plasticity at a Cortical Input to Hippocampus.
Reactivity : Rat
Tseng TL et al. Br J Pharmacol 2015; (Epub) Induction of endothelium-dependent constriction of mesenteric arteries in endotoxemic hypotensive shock.
Application : Immunoassay
Reactivity : Rat
David MD et al. J Cell Sci 2014; 127 (Pt 2):400-10 The RhoGAP ARHGAP19 controls cytokinesis and chromosome segregation in T lymphocytes.
Application : ICC/IF
Reactivity : Human
Xiao Y et al. Mol Cell Biol 2014; 14-3-3τ Promotes Breast Cancer Invasion and Metastasis by Inhibiting RhoGDIα.
Application : WB
Reactivity : Human
Chuang HH et al. Biochem J 2012; 443 (1):145-51 ROCKII Ser1366 phosphorylation reflects the activation status.
Application : WB, IHC-Fr
Reactivity : Human, Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169