Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with RPS6 antibody (GTX130450) diluted at 1:5000 and competitor's antibody (CST#2217) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

RPS6 antibody detects RPS6 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPS6 stained by RPS6 antibody (GTX130450) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.