Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 (phospho Ser235/236) antibody (GTX130430) diluted at 1:10000.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 (phospho Ser235/236) antibody (GTX130430) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 (phospho Ser235/Ser236) antibody (GTX130430) diluted at 1:10000. (CIP: Calf-intestinal alkaline phosphatase)