Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 (phospho Ser240/Ser244) antibody (GTX133942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 (phospho Ser240/Ser244) antibody (GTX133942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 (phospho Ser240/Ser244) antibody (GTX133942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.