Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RRM1 antibody (GTX100758) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

RRM1 antibody detects RRM1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
RRM1 stained by RRM1 antibody (GTX100758) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RRM1 antibody detects RRM1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RRM1 stained by RRM1 antibody (GTX100758) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RRM1(GTX100758) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

RRM1 antibody detects RRM1 protein by Western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg Raw264.7 whole cell lysate/extract
C. 30 μg C2C12 whole cell lysate/extract
5 % SDS-PAGE
RRM1 antibody (GTX100758) dilution: 1:1000

RRM1 antibody detects RRM1 protein on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
RRM1 antibody (GTX100758) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

RRM1 antibody detects RRM1 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: RRM1 protein stained by RRM1 antibody (GTX100758) diluted at 1:1000.
Blue: Hoechst 33342 staining.