Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Rab 1A antibody [N1C3] (GTX101454) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot analysis of RAB1A expression in transfected 293T cell line by RAB1A polyclonal antibody.
A: Non-transfected lysate.
B: RAB1A transfected lysate.
12% SDS PAGE
GTX101454 diluted at 1:500

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Rab 1A antibody [N1C3] (GTX101454) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Rab 1A antibody [N1C3] (GTX101454) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.