Immunohistochemical analysis of agarose-embedded zebrafish embryo, using Rab11a + Rab11b antibody (GTX127328) at 1:200 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Zebrafish tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Rab11a + Rab11b antibody (GTX127328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal PLoS Genet in 2014.PMID: 24967841