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Rabbit IgG isotype control

Rabbit IgG isotype control. GTX35035
Rabbit IgG isotype control. GTX35035
Rabbit IgG isotype control. GTX35035

Cat. No. GTX35035

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Control, IHC
Package
100 μg ($119)

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

PROPERTIES

Form

Liquid

Buffer

Borate buffered saline

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Purification

Purified by a combination of precipitation and/or affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Isotype control for Rabbit IgG , Rabbit IgG negative control , Polyclonal Rabbit IgG isotype control , Rabbit negative control for IgG , Rabbit isotype control for IgG , Rabbit IgG isotype control , Negative control for Rabbit IgG , Isotype control to Rab

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

DATA IMAGES

Rabbit IgG isotype control. GTX35035

GTX35035 FACS Image

The data was published in the journal Int J Mol Sci in 2018. PMID: 30042348

Rabbit IgG isotype control. GTX35035

GTX35035 IHC-Fr Image

The data was published in the journal PLoS One in 2017. PMID: 29095915

Rabbit IgG isotype control. GTX35035

GTX35035 IHC-P Image

The data was published in the journal Reprod Biol Endocrinol in 2013. PMID: 23849271

REFERENCE

REVIEW

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Application Reference
Package List Price ($)
$ 119