Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Rad1 antibody [4126] (GTX70317) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.