Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal (GTX119732) diluted at 1:2000.

Immunoprecipitation of Rad50 protein from NT2D1 whole cell extract using 5 μg of Rad50 antibody [C1C2], Internal (GTX119732).
Western blot analysis was performed using Rad50 antibody [C1C2], Internal (GTX119732).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Rad50(GTX119732) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: NT2D1
5% SDS PAGE
GTX119732 diluted at 1:5000

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal (GTX119732) diluted at 1:1000.

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal (GTX119732) diluted at 1:5000.