Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Mouse anti-RAD51 14B4 antibody (GTX70230) was used in IP assay (immunoprecipitation) using HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40).
Rabbit anti-RAD51 antibody (GTX100469) was used for subsequent WB detection of immunoprecipated RAD51.
Whole cell extract was used as input.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

RAD51 antibody 14B4 (GTX70230) detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
RAD51 antibody 14B4 (GTX702308) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Rad51 antibody [14B4] detects Rad51 protein at nuclear foci by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad51 stained by Rad51 antibody [14B4] (GTX70230) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Immunofluorecent staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody (GTX70230).
Cells were pre-extracted with CSK buffer before fixation with 4% PFA.
RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus.
Scale bar, 10 mciro-meter.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal Oncogene in 2019. PMID: 30177840

The data was published in the journal Oncogene in 2019. PMID: 30177840

The data was published in the journal Mol Oncol in 2017. PMID: 28173629

The data was published in the journal EMBO Mol Med in 2013. PMID: 23341130

The data was published in the journal EMBO Mol Med in 2013. PMID: 23341130

The data was published in the journal EMBO Mol Med in 2013. PMID: 23341130

The data was published in the journal Nat Commun in 2016.PMID: 26727879

The data was published in the journal Oncotarget in 2019.PMID: 31080552

The data was published in the 2017 in Cell. PMID: 28575672

The data was published in the journal Mol Oncol in 2017. PMID: 28173629

The data was published in the journal Mol Oncol in 2017. PMID: 28173629

The data was published in the journal Mol Oncol in 2017. PMID: 28173629

The data was published in the journal PLoS One in 2014. PMID: 25310191

The data was published in the journal Int J Radiat Oncol Biol Phys in 2016.PMID: 27020103

The data was published in the journal Nat Commun in 2016.PMID: 26727879

The data was published in the journal Elife in 2018.PMID: 29985131