ICC/IF analysis of GM0637 cells irradiated by X-rays (2 Gy) for 1 hr using GTX00721 Rad51 antibody.
Left : 1:100 dilution for 30 min at 37 degree C
Right : 1:1000 for 30 min at 37 degree C
Fixation : 4% PFA for 10 min
Permeabilization : 0.5% Triton X-100 for 5 min