Wild-type (WT) and RanBP1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [N1C3] (GTX103192) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Ran BP1 antibody [N1C3] detects Ran BP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ran BP1 protein stained by Ran BP1 antibody [N1C3] (GTX103192) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX103192 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: HeLa S3 (GTX14654)
12% SDS PAGE
GTX103192 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [N1C3] (GTX103192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.