Hydrolysis assay of Dns-Tyr-Val-Gly by GTX00374-pro Rat PAM protein (active) for 30 min. The substrate (Dns-Tyr-Val-Gly) have been hydrolyzed to product (Dns-Tyr-Val-NH2) when the Rat PAM protein (active) was 5 μg/ml. The retention time of Substrate and Product are 24.315 and 30.806, respectively.
Upper: Activity assay of Rat PAM protein (active) by HPLC.
Lower: The reaction products compared with standard Dns-Tyr-Val-Gly and Dns-Tyr-Val-NH2.

SDS-PAGE analysis of GTX00374-pro Rat PAM protein (active).