Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Rb antibody (GTX100545) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb antibody (GTX100545) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rb antibody detects Rb protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Rb antibody (GTX100545) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Rb antibody detects Rb protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rb stained by Rb antibody (GTX100545) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Rb antibody immunoprecipitates retinoblastoma 1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of retinoblastoma 1 protein by 4 μg Rb antibody (GTX100545)
7.5 % SDS-PAGE
The immunoprecipitated retinoblastoma 1 protein was detected by Rb antibody (GTX100545) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]