HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] (GTX70232) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

RbAp48 antibody immunoprecipitates RbAp48 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune mouse IgG B. 5 μg of RbAp48 antibody (GTX70232) The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.

Immunofluorescence analysis of HeLa, using RbAp48(GTX70232) antibody at 1:100 dilution.

SH-SY5Y whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] (GTX70232) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.