Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using antibodies as below.

Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1245] (GTX636647) (5 μg/mL)
Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1291] (GTX636705) (1 μg/mL)
Please notice that GTX636705 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636647. Please contact us for custom HRP-conjugated antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1291] (GTX636705) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1291] (GTX636705) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.