APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2
Predict Reactivity
Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Rho GDI2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949682
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rho GDP dissociation inhibitor beta , D4 , GDIA2 , GDID4 , LYGDI , Ly-GDI , RAP1GN1 , RhoGDI2
Cellular Localization
Cytoplasm
Background
Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
|
GTX103254 WB Image
Sample(30 μg whole cell lysate) A: 293T B: A431 (GTX27909) C: H1299 D: HeLa S3 (GTX14654) E: HepG2 (GTX27900) 12% SDS PAGE GTX103254 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for Rho GDI2 antibody [N1C3] (GTX103254). Be the first to share your publications with this product.
REVIEW
Rho GDI2 antibody [N1C3]
Cat. No. GTX103254
Rating
|
|
( Average 4 based on 2 users reviews)
|
Application
Western Blot(WB)
|
|
( Average 4 based on 2 users reviews)
|
Date :
Anonymous submitted on 13-Mar-2014
Application Tested :
WB
Sample Species :
Ms
Sample :
mouse fibroblast cells
Amount used :
30μg
Blocking :
3%BSA-TBS, 25°C, 1Hr
Primary Antibody :
1:3000, 4°C, 16Hr
Date :
Anonymous submitted on 04-Nov-2011
Application Tested :
WB
Sample Species :
Hu
Sample :
Breast cancer private cells
Amount used :
30μg
Blocking :
5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr