Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX100546 diluted at 1:3000

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX100546 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using RhoC(GTX100546) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

RhoC antibody detects RHOC protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: RHOC protein stained by RhoC antibody (GTX100546) diluted at 1:500.
Blue: Hoechst 33342 staining.