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Rhodopsin antibody [HL2668]

Recombinant antibody Comparison Orthogonal Overexpression
Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332
Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332
Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332
Anti-Rhodopsin antibody [HL2668] used in IHC (Paraffin sections) (IHC-P). GTX639332
Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332
Anti-Rhodopsin antibody [HL2668] used in IHC (Paraffin sections) (IHC-P). GTX639332
Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332
Anti-Rhodopsin antibody [HL2668] used in IHC (Whole mount) (IHC-Wm). GTX639332
Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332

Cat. No. GTX639332

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2668

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Rhodopsin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human Rhodopsin.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

rhodopsin , CSNBAD1 , OPN2 , RP4

Cellular Localization

Membrane

Background

The protein encoded by this gene is found in rod cells in the back of the eye and is essential for vision in low-light conditions. The encoded protein binds to 11-cis retinal and is activated when light hits the retinal molecule. Defects in this gene are a cause of congenital stationary night blindness. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332

GTX639332 WB Image

Comparison Orthogonal

Boiled and unboiled mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:1000 and competitor's antibody (Clone 1D4) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332

GTX639332 WB Image

Comparison Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:5000 and competitor's antibody (Clone 1D4) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332

GTX639332 WB Image

Orthogonal

Boiled and unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rhodopsin antibody [HL2668] used in IHC (Paraffin sections) (IHC-P). GTX639332

GTX639332 IHC-P Image

Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Rhodopsin stained by Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332

GTX639332 WB Image

Rat tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rhodopsin antibody [HL2668] used in IHC (Paraffin sections) (IHC-P). GTX639332

GTX639332 IHC-P Image

Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Rhodopsin stained by Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332

GTX639332 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rhodopsin antibody [HL2668] used in IHC (Whole mount) (IHC-Wm). GTX639332

GTX639332 IHC-Wm Image

Rhodopsin antibody [HL2668] detects Rhodopsin protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 3 days-post-fertilization zebrafish embryo.
Green: Rhodopsin stained by Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Rhodopsin antibody [HL2668] used in Western Blot (WB). GTX639332

GTX639332 WB Image

Human tissue extract was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Currently in production
Custom Conjugations