S100A4 antibody, C-term

Cat. No. GTX89197

Reference 4

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse
Package	100 μg ($399)

See all S100A4 products

View product citations for antibody GTX89197 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.05-0.2μg/ml
ICC/IF	Assay dependent
IHC-P	1-2μg/ml
IHC	Assay dependent
Note : IHC-P Human Small Intestine shows staining of lymphatic cells in the lamina propria.

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89197-PEP).

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CNEFFEGFPDKQP, from the C Terminus of the protein sequence according to NP_002952.1; NP_062427.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726478

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

S100 calcium binding protein A4 , 18A2 , 42A , CAPL , FSP1 , MTS1 , P9KA , PEL98

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Database

Gene ID: 6275 S100A4
UniProt: P26447 S100A4

Research Area

DATA IMAGES

GTX89197 WB Image

WB analysis of peripheral blood mononuclear cells lysate using GTX89197 S100A4 antibody, C-term.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer