Unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with S1PR2 antibody [HL3765] (GTX641964) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with S1PR2 antibody [HL3765] (GTX641964) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Boiled and unboiled HCT-116 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EDG5 antibody [HL3765] (GTX641964) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)