SAE1 antibody detects SAE1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SAE1 protein stained by SAE1 antibody (GTX113602) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

SAE1 antibody detects SAE1 protein by Western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
SAE1 antibody (GTX113602) dilution: 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX113602 diluted at 1:1000

Immunoprecipitation of SAE1 protein from HepG2 whole cell extracts using 5 μg of SAE1 antibody (GTX113602).
Western blot analysis was performed using SAE1 antibody (GTX113602).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using SAE1(GTX113602) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGE
GTX113602 diluted at 1:1000

The data was published in the journal Genes (Basel) in 2014.PMID: 25333844