Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] (GTX636807) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] detects SARS-CoV-2 (COVID-19) 3CLpro (nsp5) protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) 3CLpro (nsp5) stained by SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] (GTX636807) diluted at 1:500.
Blue: Hoechst 33342 staining.