SARS-CoV-2 (COVID-19) Membrane antibody [HL1087]

Name: SARS-CoV-2 (COVID-19) Membrane antibody [HL1087]
Brand: GeneTex
SKU: GTX636245
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-SARS-CoV-2 (COVID-19) Membrane antibody [HL1087] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636245

Anti-SARS-CoV-2 (COVID-19) Membrane antibody [HL1087] used in Western Blot (WB). GTX636245

Cat. No. GTX636245

Reference 1

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1087
Isotype	IgG
Application	WB, ICC/IF
Reactivity	SARS Coronavirus 2
Package	100 μl ($399), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) Membrane products

Datasheet
Reference

View product citations for antibody GTX636245 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1:100-1:2000

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of SARS-CoV-2 (COVID-19) Membrane. (Strain:Wuhan-hu-1)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909954

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636245 ICC/IF Image

SARS-CoV-2 (COVID-19) Membrane antibody [HL1087] detects SARS-CoV-2 (COVID-19) Membrane protein by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Membrane stained by SARS-CoV-2 (COVID-19) Membrane antibody [HL1087] (GTX636245) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636245 WB Image

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Membrane antibody [HL1087] (GTX636245) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Yamamoto Y et al. Int J Mol Sci 2022; 23 (24) SARS-CoV-2 Spike Protein Mutation at Cysteine-488 Impairs Its Golgi Localization and Intracellular S1/S2 Processing.

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