Sandwich ELISA detection of recombinant Spike RBD proteins of SARS-CoV-2 (COVID-19) derived from different strains (Delta variant B.1.617.2 or Wide type strain) using the antibodies as follows.
Capture : SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Contact us for custom HRP-conjugated antibody.

1 μg of GTX136332-pro SARS-CoV-2 (COVID-19) Spike RBD (L452R, T478K Mutant) protein, His tag protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD (L452R, T478K Mutant) protein, His tag protein (GTX136332-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

Inhibition analysis of immobilized recombinant RBD proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Functional ELISA analysis of immobilized recombinant Spike RBD proteins of SARS-CoV-2 (COVID-19) derived from different strains (Delta variant B.1.617.2 or Wide type strain) (2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).