Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.12.1 variant, His tag (GTX137249-pro) (143.68-2.24 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike RBD antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.12.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

GTX137249-pro SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.12.1 variant, His tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.12.1 variant, His tag (1 μg, GTX137249-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.